Journal:

Article Title: Lentiviral delivery of HIV-1 Vpr protein induces apoptosis in transformed cells

doi:

Figure Lengend Snippet: Vpr preferentially kills rapidly dividing cells. (a) Human fibroblasts were grown in Iscove’s plus 1%-depleted FCS or 10% FCS. Cells (2 × 104) were plated on day 0 and counted on days 1, 3, 5, 7, and 9. (b) Normal human fibroblasts were grown in 10% FCS and mock-infected (⧫) or infected with HIV-1NL4–3Thyenv(-) (■), HR’Thy(Vpr−) (▴), or HR’Thy(Vpr+) (×). At 144 h postinfection, infection with HIV-1NL4–3Thyenv(-), HR’Thy(Vpr−), and HR’Thy(Vpr+) resulted in 63%, 66%, and 71% Thy 1.2-positive cells, respectively. At 72, 144, and 216 h postinfection, Thy 1.2-positive cells were analyzed for annexin V staining. (c) Normal human fibroblasts were grown in 1% depleted FCS and mock-infected (⧫) or infected with HIV-1NL4–3Thyenv(-) (■), HR’Thy (Vpr−) (▴), or HR’Thy(Vpr+) (×). At 144 h postinfection, infection with HIV-1NL4–3Thyenv(-), HR’Thy(Vpr−), and HR’Thy(Vpr+) resulted in 48%, 65%, and 82% Thy 1.2-positive cells, respectively. At 72, 144, and 216 h postinfection, Thy 1.2-positive cells were analyzed for annexin V staining.

Article Snippet: Normal human diploid fibroblasts (ATCC CRL2076) were maintained in Iscove’s plus either 10% FCS or 1%-depleted FCS.

Techniques: Infection, Staining

Journal: Carcinogenesis

Article Title: Chromium(VI) exposure enhances polycyclic aromatic hydrocarbon-DNA binding at the p53 gene in human lung cells.

doi: 10.1093/carcin/bgg012

Figure Lengend Snippet: Fig. 1. The effect of Cr(VI) pre-exposure on BPDE±DNA adduct formation in exon 7 of the p53 gene in normal human lung (A) and skin (B) fibroblast cells. Cells were pre-exposed to different concentrations of K2CrO4 for 48 h and then treated with 1 mM BPDE for 30 min. Genomic DNA was isolated, and treated with UvrABC nuclease. BPDE±DNA adduct distribution was mapped by the LMPCR method as described in Materials and methods. (A and B) Typical autoradiographs; (C and D) quantifications. UvrABC nuclease incision bands corresponding to the codons of interest are labeled at the right-hand side of the panel. G, A G and T C are Maxam±Gilbert sequencing reaction. The RI of UvrABC nuclease incision bands were calculated as described in Materials and methods. Results represent three independent experiments.

Article Snippet: Cells, cell culture and Cr(VI) exposure The normal human lung fibroblast cells (CCL-202, ATCC, Manassas, VA) were grown in minimal essential medium supplemented with 10% fetal bovine serum; the normal human skin fibroblast cells (CRL-2076, ATCC) were grown in Iscove's modified Dulbecco's medium supplemented with 10% fetal bovine serum according to the vendor's instruction.

Techniques: Isolation, Labeling, Sequencing

Journal: Carcinogenesis

Article Title: Chromium(VI) exposure enhances polycyclic aromatic hydrocarbon-DNA binding at the p53 gene in human lung cells.

doi: 10.1093/carcin/bgg012

Figure Lengend Snippet: Fig. 2. The effect of Cr(VI) pre-exposure on BPDE±DNA adduct formation in exon 8 of the p53 gene in normal human lung (A) and skin (B) fibroblast cells. The same UvrABC nuclease-treated genomic DNA as described in Figure 1 was used to map BPDE±DNA adduct distribution in exon 8 of the p53 gene by the LMPCR method. (A and B) Typical autoradiographs; (C and D) quantifications. UvrABC nuclease incision bands corresponding to codons of interest are labeled at the right-hand side of the panel. A G and T C are Maxam±Gilbert sequencing reaction. The RI of UvrABC nuclease incision bands were calculated as described in Materials and methods. Results represent three independent experiments.

Article Snippet: Cells, cell culture and Cr(VI) exposure The normal human lung fibroblast cells (CCL-202, ATCC, Manassas, VA) were grown in minimal essential medium supplemented with 10% fetal bovine serum; the normal human skin fibroblast cells (CRL-2076, ATCC) were grown in Iscove's modified Dulbecco's medium supplemented with 10% fetal bovine serum according to the vendor's instruction.

Techniques: Labeling, Sequencing

Journal: Carcinogenesis

Article Title: Chromium(VI) exposure enhances polycyclic aromatic hydrocarbon-DNA binding at the p53 gene in human lung cells.

doi: 10.1093/carcin/bgg012

Figure Lengend Snippet: Fig. 3. The effect of Cr(VI) pre-exposure on BPDE±DNA adduct formation in exon 5 of the p53 gene in normal human lung and skin fibroblast cells. The same UvrABC nuclease-treated genomic DNA as described in Figures 1 and 2 was used to map BPDE±DNA adduct distribution in exon 5 of the p53 gene by the LMPCR method. (A and B) Typical autoradiographs; (C and D) quantifications. UvrABC nuclease incision bands corresponding to the codons of interest are labeled at the right-hand side of the panel. G, A G and T C are Maxam±Gilbert sequencing reaction. The RI of UvrABC nuclease incision bands was calculated as described in Materials and methods. Results represent three independent experiments.

Article Snippet: Cells, cell culture and Cr(VI) exposure The normal human lung fibroblast cells (CCL-202, ATCC, Manassas, VA) were grown in minimal essential medium supplemented with 10% fetal bovine serum; the normal human skin fibroblast cells (CRL-2076, ATCC) were grown in Iscove's modified Dulbecco's medium supplemented with 10% fetal bovine serum according to the vendor's instruction.

Techniques: Labeling, Sequencing

Journal: Carcinogenesis

Article Title: Chromium(VI) exposure enhances polycyclic aromatic hydrocarbon-DNA binding at the p53 gene in human lung cells.

doi: 10.1093/carcin/bgg012

Figure Lengend Snippet: Fig. 4. The effect of Cr(VI) exposure on BPDE±DNA adduct formation in exon 6 of the p53 gene in normal human lung (A) and skin (B) fibroblast cells. The same UvrABC nuclease-treated genomic DNA as described in Figures 1 and 2 was used for mapping BPDE±DNA adduct distribution in exon 6 of the p53 gene by the LMPCR method. UvrABC nuclease incision bands corresponding to the codons of interest are labeled at the right-hand side of the panel. A G and T C are Maxam±Gilbert sequencing reaction.

Article Snippet: Cells, cell culture and Cr(VI) exposure The normal human lung fibroblast cells (CCL-202, ATCC, Manassas, VA) were grown in minimal essential medium supplemented with 10% fetal bovine serum; the normal human skin fibroblast cells (CRL-2076, ATCC) were grown in Iscove's modified Dulbecco's medium supplemented with 10% fetal bovine serum according to the vendor's instruction.

Techniques: Labeling, Sequencing

Journal: Carcinogenesis

Article Title: Chromium(VI) exposure enhances polycyclic aromatic hydrocarbon-DNA binding at the p53 gene in human lung cells.

doi: 10.1093/carcin/bgg012

Figure Lengend Snippet: Fig. 5. The effect of Cr(VI) pre-exposure on total BPDE±DNA adduct formation at the genomic DNA level in normal human lung and skin fibroblast cells. Cells were pre-exposed to K2CrO4 for 48 h, and then treated with [3H]BPDE for 30 min. The genomic DNA was isolated, and the [3H] d.p.m. of a known amount of purified genomic DNA was determined by a scintillation counter. The number of BPDE±DNA adducts in 10 kb genomic DNA was calculated based on the specific activity of the [3H]BPDE as described in Materials and methods. Results represent three independent experiments.

Article Snippet: Cells, cell culture and Cr(VI) exposure The normal human lung fibroblast cells (CCL-202, ATCC, Manassas, VA) were grown in minimal essential medium supplemented with 10% fetal bovine serum; the normal human skin fibroblast cells (CRL-2076, ATCC) were grown in Iscove's modified Dulbecco's medium supplemented with 10% fetal bovine serum according to the vendor's instruction.

Techniques: Isolation, Purification, Activity Assay

Journal: Carcinogenesis

Article Title: Chromium(VI) exposure enhances polycyclic aromatic hydrocarbon-DNA binding at the p53 gene in human lung cells.

doi: 10.1093/carcin/bgg012

Figure Lengend Snippet: Fig. 6. BPDE±DNA adduct distribution in exons 7 (A) and 8 (B) of the p53 gene in BPDE-modified genomic DNA isolated from normal human lung fibroblast cells with and without Cr(VI) pre-exposure. Cells were pre-exposed to K2CrO4 for 48 h, and genomic DNA was isolated. Purified genomic DNA was modified with 1 mM BPDE in vitro and BPDE±DNA adduct distribution in exons 7 and 8 of the p53 gene was mapped by the the UvrABC nuclease incision in combination with the LMPCR method. UvrABC nuclease incision bands corresponding to the codons of interest are labeled at the right-hand side of the panel. A G and T C are Maxam±Gilbert sequencing reaction.

Article Snippet: Cells, cell culture and Cr(VI) exposure The normal human lung fibroblast cells (CCL-202, ATCC, Manassas, VA) were grown in minimal essential medium supplemented with 10% fetal bovine serum; the normal human skin fibroblast cells (CRL-2076, ATCC) were grown in Iscove's modified Dulbecco's medium supplemented with 10% fetal bovine serum according to the vendor's instruction.

Techniques: Modification, Isolation, Purification, In Vitro, Labeling, Sequencing

Journal: Materials

Article Title: Advancing Antimicrobial Textiles: A Comprehensive Study on Combating ESKAPE Pathogens and Ensuring User Safety

doi: 10.3390/ma17020383

Figure Lengend Snippet: ( A ) Dose-response curves of human dermal fibroblast (HDF) cell viability measured by resazurin assay of zinc chloride, silver nitrate, HM4005, HM4007, and tea tree oil solution after 1 day of exposure. Data show mean ± SE. ( B ) Microscopic bright-field images of control HDF and HDF cultures after 1 day of exposure to different concentrations of zinc chloride, silver nitrate, HM4005, HM4007, and tea tree oil solutions. Scale bars = 100 μm.

Article Snippet: Normal human adult dermal fibroblast cells (HDFs; line CCD-1064Sk—ATCC CRL-2076) were used as a model of dermis cells.

Techniques: Resazurin Assay, Control

Journal: Materials

Article Title: Advancing Antimicrobial Textiles: A Comprehensive Study on Combating ESKAPE Pathogens and Ensuring User Safety

doi: 10.3390/ma17020383

Figure Lengend Snippet: Biocompatible assessment of tested antimicrobial agents. ( A ) Images of leachates prepared from textiles coated with the different antimicrobial agents. Human dermal fibroblast (HDF) cell viability measurements using resazurin assay ( B ) and MTT assay ( C ). Whisker plots show HDF cell viability after 1 day of exposure with the suspension from antimicrobial-agent-impregnated textiles from different fabric–media incubation periods. Data show mean ± SE, * p < 0.05, ** p < 0.01, *** p < 0.0002, and **** p < 0.0001, compared to untreated HDF cells.

Article Snippet: Normal human adult dermal fibroblast cells (HDFs; line CCD-1064Sk—ATCC CRL-2076) were used as a model of dermis cells.

Techniques: Resazurin Assay, MTT Assay, Whisker Assay, Suspension, Incubation